Detection of Biofilm Formation by Escherichia coli and Staphylococcus aureus Associated with Canine Pyometra

Background: Antibiotic resistance is one of the major problems encountered in therapy canine pyometra. The ability bacteria to form biofilm implicated as factors responsible for this. Escherichia coli and Staphylococcus aureus are predominant associated with pyometra canines known their formation. Keeping this view, a preliminary study was conducted detect forming strains E. S. aureus, if any, Methods: A total 25 samples were collected, which included uterine discharges from cases closed anterior vaginal swabs open isolates identified based on cultural, morphological biochemical characteristics. These subjected antibiotic sensitivity test employing disc diffusion method. For detection, screened by Congo red agar method, tube method tissue culture plate Result: From samples, two Streptococcus spp., thirteen seven E.coli, five Klebsiella spp. Pseudomonas isolated. All found be multi-drug resistant antibiogram. more sensitive formation E.coli isolates, respectively. showed higher degree comparison non-formers, indicating it reasons failure